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Characterization of ten microsatellite loci in the blue mussel Mytilus edulis ArchiMer
Lallias, Delphine; Stockdale, Ruth; Boudry, Pierre; Lapegue, Sylvie; Beaumont, Andy R..
Mussels of the genus Mytilus are ecologically and commercially important worldwide, and they form hybrid complexes where their distributions overlap. Allozyme and nuclear markers have bee It Used to investigate their genetics over many years, but successful development of reliable highly valuable microsatellite markers has lagged be hind other shellfish species. We have developed and characterized tell novel microsatellite loci that amplify reliably for the blue mussel Mytilus edulis. The number of alleles among 30 individuals from a wild population (Menai Strait, North Wales UK) ranged between 9 and 29 and the observed heterozygosity between 0.300 and 0.954. Significant heterozygote deficiencies against the Hardy-Weinberg model were observed at 6 out of...
Tipo: Text Palavras-chave: Mytilus edulis; Microsatellites; Universal tailed-primer labeling; Null alleles; Mussels.
Ano: 2009 URL: http://archimer.ifremer.fr/doc/00002/11275/7841.pdf
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Distribution of glutathione S-transferase GSTM1 and GSTT1 null phenotypes in Brazilian Amerindians Genet. Mol. Biol.
Klautau-Guimarães,Maria de Nazaré; Hiragi,Cássia de Oliveira; D'Ascenção,Renata Ferreira; Oliveira,Silviene Fabiana; Grisolia,Cesar Koppe; Hatagima,Ana; Ferrari,Íris.
The distribution of glutathione S-transferase (GST) GSTM1 and GSTT1 null phenotype frequencies in two Brazilian Amerindian tribes, the Munduruku tribe from Missão Cururu village (79 individuals) and the Kayabi tribe (41 individuals), was analyzed by polymerase chain reaction (PCR) amplification. The GST null phenotype frequencies for the Munduruku sample were 0% for GSTM1 and 27% for GSTT1 while for the Kayabi sample the null phenotype frequencies were 27% for GSTM1 and 29% for GSTT1. This is the first report of the absence of the GSTM1 null phenotype in any ethnic group.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Munduruku; Glutathione S-transferase; Kayabi; Null alleles.
Ano: 2005 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572005000100005
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Identification and characterization of 18 novel polymorphic microsatellite makers derived from expressed sequence tags in the Pacific oyster Crassostrea gigas ArchiMer
Sauvage, Christopher; Boudry, Pierre; Lapegue, Sylvie.
We report the development of 18 new polymorphic microsatellite DNA markers derived from Crassostrea gigas expressed sequences tags. Genotyping of 48 wild adult oysters sampled from Marennes-OlEron bay (France) revealed 12 to 48 alleles per locus. Observed and expected heterozygosity levels ranged from 0.64 to 1 and from 0.77 to 0.97, respectively. The development of these new markers creates a useful complementary tool for population genetics studies, parentage analysis and mapping in Pacific oyster, a species of major aquacultural and ecological importance.
Tipo: Text Palavras-chave: Pacific oyster; Null alleles; EST SSR markers; Crassostrea gigas.
Ano: 2009 URL: http://archimer.ifremer.fr/doc/2009/publication-6419.pdf
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